NycoCard Reader II; Features and applications

NycoCard II assays

The Abbott NycoCard READER II user Manual pdf includes all of the steps necessary to make it simple to use for all users. nycocard reader ii service manual comes with the device and is also available as a PDF online for download. the Available tests carried out by the device include:

1- HbA1c test

Glycated hemoglobin, also known as HbA1c or simply A1c, measurements provides an accurate and dependable method of routinely monitoring the quality of metabolic control in diabetics.
Several studies have shown that good metabolic control is important in preventing and slowing the progression of diabetic late complications in people with type I and type II diabetes.

Characteristics of NycoCard HbAc

• Sample material: 5μl Whole blood
• Test result within 3 minutes
• Measuring range: 3-18% HbAc
• Reference range: 4.5-6.3% Hb1Ac
• No interference of hemoglobin variants of derivatives
• Within and between runs, the coefficient of variation is less than 5%.

Test procedure

1. Mix 5 μl whole blood into the R1 reagent. Mix thoroughly. incubate for 2-3 minutes
2. Remix. Apply 25 μl of the reaction mixture to the Test Device. Allow soaking
3. Pour in 25 μl of R2/Washing Solution. Allow soaking
4. Using the NycoCard READER, read the result within 5 minutes.

2- CRP test

NycoCard measures C-reactive protein (CRP), which is an acute phase protein that rises rapidly after infection, Importantly, high readings are usually seen in bacterial infections, but only low to moderate levels in viral infections.

Characteristics of Nycocard CRP

• Measuring range
  • Whole blood samples: 8-250 mg/L
  • Serum or plasma sample: 5-150 mg/L
• Sample Material: 5 μl of whole blood/serum/plasma
• Test Principle: Solid phase, sandwich-format, an immunometric assay using a gold antibody conjugate.
• Expected values: Normal values < 5mg/L

Procedure for testing

1.  Place a 5 l capillary containing the patient sample into the R1/Dilution Liquid tube and mix thoroughly for 10 seconds.
2.  Fill the test device with 50 l of diluted sample. Allow time to soak (approx 30 seconds).
3.  Conjugate the test device with 1 drop R2. Allow time to soak (approx 30 seconds).
4.  Wet the test device with 1 drop of R3/Washing Solution. Allow time to soak (approx 20 seconds).
5. Read the outcome in 5 minutes. For blood samples, use the CRP Whole Blood menu, and for serum plasma samples, use the Serum/Plasma menu.

3- D-Dimer test

D-Dimer is a protein that is released into the bloodstream during the breakdown of a fibrin blood clot.
The presence of D-Dimer in the bloodstream is used to detect the formation and breakdown of a blood clot somewhere in the body.

Characteristics of Nycocard ii D-Dimer

• Accurate D-dimer results within two minutes
• Combines the speed and simplicity of the latex methods with the analytical quality of ELISA methods
• No dilution of plasma · Defined positive control included in the kit
• Available both as card and single test ·No time-dependent incubation step
• Sample Material: 50 μl of undiluted platelet-free citrated plasma
• Test Principle: Solid phase, sandwich-format, an immunometric assay using a gold antibody conjugate
• Measuring range: 0.1-20mg/L
• Expected values: Normal values < 0.3mg/L

Test procedure

1.  Fill the test device with 50 μl of R2/washing solution. Allow time to soak
2. Fill the device with 50 μl of platelet-free citrated plasma. Allow the sample to soak for a few minutes (approx 50 seconds)
3. Pour 50 μl of R1/conjugate into the device and soak it (approx 50 seconds).
4. Pour 50 µl of R2/washing solution into the test device and soak for 30 minutes
5. Read the result within 2 minutes using Nycocard READER ii

4- µ-Albumin (Microalbumin)

Microalbuminuria is defined as a persistent increase in urinary albumin excretion to 20-200 g/min or 20-200 mg/L when using early morning urine. Microalbuminuria has been shown in studies to independently predict cardiovascular morbidity and all-cause mortality in people with essential hypertension. Monitoring µ-Albumin is worthwhile in order to assess the effect of antihypertensive treatment on target-organ damage.

Characteristics NyoCard µ-Albumin

• simply quick and convenient – within only 3 minutes a quantitative test result is obtained
• Sample Material: 50μl urine
• Measuring range: 5-200 mg per L albumin
• Rest principle: Solid phase, sandwich-format, immunometric assay
  using a gold-antibody conjugate.
• In controlled laboratory testing, a coefficient of variation(CV) of 5-8% is usually obtained