Understanding the Urinary Urobilinogen Test: Overview, Purpose, Procedure, and Results

The Urine Urobilinogen Test is a diagnostic tool used to detect liver and bile duct diseases. Learn more about this important test and its significance in this article.

The Urine Urobilinogen Test is a medical test that can help diagnose liver and bile duct diseases. This test measures the amount of urobilinogen, a substance produced by the liver, in a person’s urine. In this article, we’ll explore the significance of this test and what it can tell us about a person’s health.


Test Overview

The urinary urobilinogen test is a urine analysis that measures the amounts of urobilinogen in the urine sample. Urobilinogen is a byproduct of the breakdown of hemoglobin, a protein found in red blood cells that carries oxygen throughout the  body. After hemoglobin is broken down, urobilinogen is formed in the liver and excreted into the small intestines, where it is converted into other substances that are eventually eliminated from the body in the stool.

A small amount of urobilinogen is also excreted in the urine, and the urinary urobilinogen test measures the level of urobilinogen in a urine sample to evaluate liver and kidney function.

The test can be performed using a random urine sample or a 24-hour urine collection. A healthcare provider may order the test if they suspect that an individual has liver disease, kidney disease, or hemolytic anemia. The test may also be ordered to monitor the progression of a known medical condition or to evaluate the effectiveness of treatment.

Interpretation of the urinary urobilinogen test results will depend on the individual’s medical history, physical examination, and other laboratory test results. The healthcare provider will determine whether the results are within the normal range and whether further testing or treatment is necessary.

Table: Urobilinogen Test Description

Test Name Uobilinogen Test in Urine
Purpose To measure the amount of urobilinogen in urine
Normal Range Less than 1 mg/dL or 17 µmol/L
High Levels Indicate Liver disease, hepatitis, hemolytic anemia
Low Levels Indicate Bile duct blockage, liver dysfunction
Procedure Collect urine sample
Preparations Clean genital area before collecting sample, avoid certain medications
Results Reported in mg/dL or µmol/L

Why is the Urine Urobilinogen Test Necessary?

Urine Urobilinogen Test
Urobilinogen Test in Urine

The urine urobilinogen test is usually done as part of a routine urinalysis. It can help detect problems with the liver, bile ducts, and red blood cells. Abnormal levels of urobilinogen can indicate liver disease, hepatitis, or hemolytic anemia. Additionally, the test can help monitor the effectiveness of treatment for these conditions.


Symptoms requiring performing this test

The Uobilinogen Test in Urine may be ordered by a healthcare provider if an individual is experiencing symptoms that may be related to liver or kidney problems, such as:

  • Jaundice, which is a yellowing of the skin and eyes.
  • Abdominal pain or swelling.
  • Fatigue or weakness.
  • Loss of appetite or nausea.
  • Dark urine or pale stools.
  • Itching or rash.
  • Unexplained weight loss.
  • Blood in the urine.

These symptoms can indicate various medical conditions, including liver disease, kidney disease, or hemolytic anemia. The urobilinogen test, along with other laboratory tests, can help healthcare providers evaluate the function of the liver and kidneys and determine the cause of these symptoms.

It’s important to note that not everyone with these symptoms will require a urobilinogen test, and the decision to order the test will depend on the individual’s medical history, physical examination, and other laboratory test results. Therefore, it’s important to discuss any concerning symptoms with a healthcare provider.


What Happens During the Test?

The Uobilinogen Test in Urine is a quick and painless procedure that involves collecting a urine sample (pee). The healthcare provider may ask the patient to provide a “clean catch” urine sample, which involves cleaning the genital area before urinating into a sterile container. Alternatively, a urine collection bag may be used for infants and young children.


Preparations Before Uobilinogen Test in Urine

Follow the healthcare instructions, where the patient may be asked to refrain from taking certain medications before the test. These include antibiotics, diuretics, and sulfonamides. The healthcare provider may also advise the patient to avoid eating foods that can affect the color of the urine, such as beets and rhubarb.


What Do the Test Results Mean?

The results of the urobilinogen test are reported in mg/dl or µmol/L. In general, a normal range for urobilinogen in urine is 0.2 to 1.0 mg/dL, or less than 17 µmol/L.

The normal range of urobilinogen in urine can vary slightly depending on the laboratory that performs the test.
Abnormal results of the urobilinogen test may indicate a problem with the liver or kidneys. elevated urobilinogen in urine may be a sign of liver disease, such as hepatitis or cirrhosis, or hemolytic anemia, which is a condition where red blood cells are destroyed too quickly. increased urobilinogen can also occur with biliary tract obstruction, which is a blockage in the bile ducts that can lead to jaundice.

On the other hand, decreased urobilinogen in urine may be a sign of a problem with the liver or bile ducts, or it can be a result of certain medications or a genetic condition called Dubin-Johnson syndrome.

It’s important to note that the interpretation of the Urobilinogen Test in Urine results should be done by a healthcare provider in the context of the individual’s medical history and other laboratory test results. Therefore, it’s essential to discuss the results with a healthcare provider to determine the cause of abnormal urobilinogen levels and any necessary treatment or follow-up.

“Related: Urine Blood Test: Importance, and Implications of Results”


Related tests

The Urine Urobilinogen Test is often performed alongside other laboratory tests to evaluate liver and kidney function or diagnose certain medical conditions. Some related tests include:

  1. Liver function tests (LFTs): LFTs measure the levels of enzymes and other substances in the blood that are produced by the liver. Abnormal results can indicate liver damage or disease.
  2. Complete blood count (CBC): The CBC measures the number and type of blood cells in the body, including red blood cells, white blood cells, and platelets. Abnormal results can indicate various medical conditions, such as anemia, infection, or cancer.
  3. Creatinine and Blood Urea Nitrogen (BUN) tests: These tests measure the levels of creatinine and BUN in the blood, which can help evaluate kidney function.
  4. Bilirubin test: Bilirubin is a waste product produced by the liver when it breaks down old red blood cells. Abnormal bilirubin levels in the blood can indicate liver or bile duct problems, such as hepatitis or gallstones.
  5. Urinalysis: Urinalysis is a test that examines a urine sample for the presence of abnormal cells, bacteria, or other components of urine. Abnormal results can indicate various medical conditions, such as a urinary tract infection or kidney disease.

It’s important to note that the doctors will determine which tests are necessary based on the individual’s medical history, symptoms, and physical examination.


The Urine Urobilinogen Test is a simple and non-invasive way to measure the amount of urobilinogen in urine. It can help detect liver disease, hepatitis, hemolytic anemia, and problems with the bile ducts. Patients should be aware of any medications or foods they should avoid before the test. The test results can provide valuable information about the patient’s overall health and the effectiveness of treatment for certain conditions.

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